Erratum: Single-nucleotide polymorphisms of matrix metalloproteinase genes are associated with graft fibrosis after kidney transplantation
Erratum to: Transl Androl Urol 2023;12:375-83.
In the article (1) entitled “Single-nucleotide polymorphisms of matrix metalloproteinase genes are associated with graft fibrosis after kidney transplantation” (Transl Androl Urol 2023;12:375-83), one image of Figure 2D selected to represent the motility ability of HK2 cells treated with the MMP-9 rs9509 mutant plasmid was incorrect, the scale bar of Figure 2D,2E, and the figure legends of Figure 2A,2C-2E was incorrect.
The original Figure 2 and Figure 2’s legend are presented below:
Figure 2 Biological identification of the MMP-9 rs9509 mutant plasmid in the HK2 cells. (A) The HK2 cells were treated with TGF-β1, and the expression of MMP9 was examined by western blotting assays. (B) Identification of the MMP9 rs9509 mutant plasmid in the HK2 cells. ***, P<0.001. (C) The HK2 cells were treated with TGF-β1 and/or the MMP9 rs9509 mutant plasmid, while the biomarkers related to EMT were examined by western blotting assays. (D) Transwell assays were used to examine the biological function of the MMP9 rs9509 mutant plasmid. The cells were stained with 0.1% crystal violet, scale bar: 20 µm. (E) Wound-healing assays were used to examine the motility function of the MMP9 rs9509 mutant plasmid. The scratch was photographed under a light microscope at ×100 magnification, scale bar: 20 µm. TGF-β, transforming growth factor-β; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; α-SMA, α-smooth muscle actin; FN, fibronectin; EMT, epithelial-mesenchymal transition.
The correct version of Figure 2, containing the correct figure of transwell results of HK2 cells treated with plasmids for 24 hours, and the correct version of Figure 2’s legend are shown below:
Figure 2 Biological identification of the MMP-9 rs9509 mutant plasmid in the HK2 cells. (A) The HK2 cells were treated with TGF-β, and the expression of MMP9 was examined by western blotting assays. (B) Identification of the MMP9 rs9509 mutant plasmid in the HK2 cells. ***, P<0.001. (C) The HK2 cells were treated with TGF-β and/or the MMP9 rs9509 mutant plasmid, while the biomarkers related to EMT were examined by western blotting assays. (D) Transwell assays were used to examine the biological function of the MMP9 rs9509 mutant plasmid. The cells were stained with 0.1% crystal violet, scale bar: 50 µm. (E) Wound-healing assays were used to examine the motility function of the MMP9 rs9509 mutant plasmid. The scratch was photographed under a light microscope at ×100 magnification, scale bar: 50 µm. TGF-β, transforming growth factor-β; MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; α-SMA, α-smooth muscle actin; FN, fibronectin; EMT, epithelial-mesenchymal transition.
The authors regretted the error and confirmed it did not significantly affect either the results or the conclusions of the paper.
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References
- Zhang H, Gao X, Gui Z, et al. Single-nucleotide polymorphisms of matrix metalloproteinase genes are associated with graft fibrosis after kidney transplantation. Transl Androl Urol 2023;12:375-83. [Crossref] [PubMed]
