Printed Abstracts
AB070. Roles and mechanisms of soybean isoflavones in androgen-independent transformation of prostate cancer
Li Xu1,2,3, Zhigang Cao1,2,3, Yemin Xue1,2,3, Shuli Zhao1,2,3, Kai Wang1,2,3, Chao Jiang1,2,3, Qichao Chen1,2,3
1Department of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing 211166, China;2Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China;3Central Laboratory, Nanjing First Hospital, Nanjing Medical University, Nanjing 211166, China
Abstract: The aims of this study were to investigate the impact of soybean isoflavones (SIFs) on the cellular oxidative stress levels in hormone-sensitive type (LNCap) and hormone-insensitive type (DU145) of prostate cancer, and to elucidate the main components and their roles in the transformation of androgen dependency (AD)/androgen independency (AI). The LNCap and DU145 cells were treated with different concentrations of SIFs monomer (including daidzin, genistin, daidzein, and genistein), and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were then measured. The content of reactive oxygen species (ROS) in these two cells was also determined after treated with certain effective monomer. The contents of MDA in the two kinds of cells treated with genistein and daidzein were increased, but the contents of SOD were decreased in a concentration-dependent manner. There was no significant difference between daidzin and genistin. The content of MDA in the DU145 cells was higher than that in the LNCap cells under the same conditions, but the content of SOD was decreased, indicating that the oxidative stress level in DU-145 was higher than LNCap, and the ROS level in DU145 treated with effective component was significantly higher than that in LNCap. Genistein and daidzein in SIFs can affect the apoptosis and proliferation of prostate cancer cells through increasing their oxidative stress levels, thus inhibit the transformation of prostate cancer toward androgen-independent type.
Keywords: Soybean isoflavones (SIFs); androgen-independent; prostate cancer
doi: 10.21037/tau.2017.s070
Cite this abstract as: Xu L, Cao Z, Xue Y, Zhao S, Wang K, Jiang C, Chen Q. Roles and mechanisms of soybean isoflavones in androgen-independent transformation of prostate cancer. Transl Androl Urol 2017;6(Suppl 3):AB070. doi: 10.21037/tau.2017.s070
