AB078. Knockdown of SPC25 inhibits cell proliferation and cycle progression in prostate cancer

Abstract: Prostate cancer (PCa) was one of the most commonly diagnosed malignant cancers in males in China. Cell-cycle aberration was a hallmark of cancer. SPC25, a component of Ndc80 complex, played an important role in regulating mitotic chromosome segregation. However, the functional roles of SPC25 in prostate cancer were still poorly understood. Our study showed for the first time that SPC25 was significantly upregulated in prostate cancer. To explore the molecular function roles of SPC25, we performed loss of function assay and found SPC25 knockdown inhibited cell proliferation and induced the decrease in S phase and the increase in G2/M phase. Furthermore, SPC25 knockdown promoted apoptosis of prostate cancer cells. Of note, bioinformatics analysis also revealed multiple functional roles of SPC25 in regulating cell proliferation, apoptosis, invasion, role of tissue factor in cancer, TGF-β signaling, and sumoylation pathway in PCa. Here, we also evaluated possible prognostic value of SPC25 using TCGA RNA-seq data and we found showed SPC25 was upregulated in high pathology stage PCa. Kaplan-Meier analysis showed that Lower SPC25 expression level was associated with better survival of PCa patients. All these results suggest that SPC25 play an oncogenic role in PCa and could act as a novel diagnostic and therapeutic target for prostate cancer.

Keywords: Prostate cancer (PCa); SPC25; prognosis; proliferation; cell cycle