AB97. MicroRNA124 regulate cell growth of prostate cancer cells by targeting iASPP
Abstract publication Urology

AB97. MicroRNA124 regulate cell growth of prostate cancer cells by targeting iASPP

Jun Chen, Hengjun Xiao, Zhansen Huang, Zhiming Hu, Tao Qi, Bin Zhang, Xin Tao, Song-Hao Liu

Department of Infertility and Sexual Medicine, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China; Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China; Department of Urology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510630, China

Abstract: Protein phosphatase1, regulatory subunit 13 like PPP1R13L, also coined iASPP, was found high expression in prostate cancer tissues and cell lines. In previous research, in vitro and in vivo RNAi mediated by artificial lentiviral shRNAs which proved that suppression of iASPP decrease the proliferation of cancer cells. Endogenous interference RNAs, microRNAs play key roles in cell proliferation by post-transcriptional regulation of gene expression. Natural base pair matched microRNA for iASPP is mir124, which was found high expression in growth factor-loss prostate cancer cell lines. In this study we examined effect of mir124 upon iASPP and proliferation of prostate cells in vitro with lentiviral infection and use artificial shRNA as control. In vitro reporter assay confirmed that mir124 binding the 3’UTR of iASPP and suppress mRNA expression. Lentivirus mediated mir124 expression decreased the proliferation and viability of PC3 while endogenous iASPP were knocked down.

Keywords: Mir124; iAsPP; prostate cancer; cell growth

doi: 10.3978/j.issn.2223-4683.2014.s097

Cite this article as: Chen J, Xiao H, Huang Z, Hu Z, Qi T, Zhang B, Tao X, Liu SH. MicroRNA124 regulate cell growth of prostate cancer cells by targeting iASPP. Transl Androl Urol 2014;3(S1):AB97. doi: 10.3978/j.issn.2223-4683.2014.s097

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