AB190. The experimental study of in vitro differentiation of human adipose derived stem cells into hematopoietic progenitor cells

Objective: To explore the in vitro culture method of inducing human adipose derived stem cells (ADSCs) differentiation into hematopoietic progenitor cells (HPCs).

Methods: Using special modified serum-free medium for in vitro induction of ADSCs differentiation into HPCs, and using fluorescence activated cell sorter (FACS), reverse transcription polymerase chain reaction (RT-PCR) and morphological observation methods for preliminary identification of the biological characteristics of induced ADSCs cells. Results: The expression levels of positive markers of HPCs concluding CD34, CD45, KDR and CD105 are significantly higher in induced ADSCs group than that in uninduced ADSCs group, and ADSCs formed sphere clusters from adherent cells after induction. Conclusions: The experimental procedures of in vitro culture method can preliminary induce ADSCs differentiation into HPCs using the special modified serum-free medium.

Keywords: Culture in vitro; adipose derived stem cells; hematopoietic progenitor cells; induced differentiation