AB223. Expression of tight junction proteins in rat vagina
Abstract publication Basic research

AB223. Expression of tight junction proteins in rat vagina

Kyung Jin Oh, Hyun-Suk Lee, Ho Suck Chung, Kyu Youn Ahn, Kwangsung Park

Department of Urology and Anatomy, BK21 PLUS Center for Creative Biomedical Scientists, Chonnam National University Medical School, Sexual Medicine Research Center, Chonnam National University, Gwangju, Korea

Aim: Tight junction plays a role in apical cell-to-cell adhesion and epithelial polarity. In this study, we investigated the expression of tight junction proteins, such as Claudin-1, zonula occludens (ZO)-1, junction adhesion molecule (JAM)-A, and occludin in rat vagina.

Methods: Female Sprague-dawley rats (230-240 g, n=20) were divided into two groups: control (n=10) and bilateral ovariectomy (n=10). The expression and cellular localization of claudin-1, ZO-1, JAM-A, and occludin were determined in each group by immunohistochemistry and Western blot.

Results: Immunolabeling of ZO-1 was mainly expressed in the capillaries and venules of the vagina. Claudin-1, JAM-A, and occludin were expressed in the epithelium of the vagina. The immunoreactivity and protein expression of claudin-1 was significantly decreased in the ovariectomy group compared with the control group.

Conclusions: Our results suggest that tight junction proteins may have an important role in the vagina. Further studies are needed to clarify the role of each tight junction protein on vaginal lubrication.

Keywords: Tight junction proteins; rat vagina; expression

doi: 10.3978/j.issn.2223-4683.2014.s223

Cite this article as: h KJ, Lee HS, Chung HS, Ahn KY, Park K. Expression of tight junction proteins in rat vagina. Transl Androl Urol 2014;3(S1):AB223. doi: 10.3978/j.issn.2223-4683.2014.s223

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