Moderated Poster Presentation
AB179. CMTM3 is reduced in prostate cancer and inhibits migration, invasion and growth of LNCaP cells
Fengzhan Hu1,2, Wanqiong Yuan1,2, Xiaolin Wang1,2, Zhengzuo Sheng1,2, Yeqing Yuan1,2, Caipeng Qin1,2, Chongjun He1,2, Tao Xu1,2
1Peking University People’s Hospital, Beijing 100044, China; 2School of Basic Medical Sciences, Peking University, Beijing 100083, China
Objective: A novel tumor suppressor gene (TSG) CKLF-like MARVEL transmembrane domain-containing member 3 (CMTM3) is reduced or undetectable in many kinds of cancers and relates tumor malignant features. We detected its role in prostate cancer for possibility of target therapy as accumulating evidence has shown that CMTM3 is a promising TSG for gene therapy.
Methods: The expression of CMTM3 detected in prostate tissue microarray, specimens and cell lines were evaluated by immunohistochemistry and semi-quantitative PCR and Western blot, respectively. After being transfected with CMTM3 adenovirus or vector (mock), the proliferation and migration and invasion of LNCaP cells were detected by transwell assay and matrigel assay, respectively. Furthermore, the effects of CMTM3 on tumor growth were performed in nude mice xenograft in vivo.
Results: We found CMTM3 was reduced in PCa tissues and cells, compared with BPH tissues, and its expression in PCa tissues was related to the Gleason score. Moreover, after being transfected with adenovirus, ectopic expression of CMTM3 in LNCaP cells led to significant inhibition of cell proliferation and migration and invasion compared with the control (P<0.05), which may be attributed to decreased Erk1/2 activity as p-Erk1/2 was remarkably reduced when CMTM3 was overexpressed. Finally, restoration of CMTM3 significantly suppressed xenograft tumor growth in vivo (P<0.01).
Conclusions: CMTM3 is reduced in prostate cancer and acts an important role in vivo and ex vivo. Prostate cancer is an urgently need to be cured disease in man’s urogenital system. CMTM3 maybe one of the key point worth further research and application.
Keywords: CKLF-like MARVEL transmembrane domain-containing member 3 (CMTM3); MARVEL; tumor suppressor gene (TSG); gene therapy
doi: 10.3978/j.issn.2223-4683.2015.s179
Cite this abstract as: Hu F, Yuan W, Wang X, Sheng Z, Yuan Y, Qin C, He C, Xu T. CMTM3 is reduced in prostate cancer and inhibits migration, invasion and growth of LNCaP cells. Transl Androl Urol 2015;4(S1):AB179. doi: 10.3978/j.issn.2223-4683.2015.s179
