AB105. The study of erectile changes of abnormal balgam syndrome with impotence rats model and the gonad axial mechanism

Abstract: To study the erectile changes of abnormal balgam syndrome with impotence rats model and the gonad axial mechanism. The study selected 70 normal mature male SD rats, 10 of them were randomly chosen as the control group, other 60 were treated with spinach and coriander diet in a cold and humid environment until the model of abnormal balgam syndrome was established, then through APO erectile experiment, the rats were randomly divided into abnormal balgam syndrome model group, medication intervention group of abnormal balgam syndrome model, abnormal balgam syndrome with impotence disease model group and medication intervention group of abnormal balgam syndrome with impotence disease model. After 3 weeks Yimusake intervention, we detected the morphological changes of penile tissue. The results showed: (I) APO erectile tests: after APO (85 μg/kg) injection, 30 min sexual behavior test founded, compared with normal control group, decreased number of erectile were observed in abnormal phlegmatic syndrome model group, but the difference have no statistical significance (P>0.05), abnormal phlegmatic syndrome and ED group reduced significantly (P<0.05); compared with abnormal phlegmatic syndrome model group, abnormal phlegmatic syndrome and ED group improved significantly (P<0.05). (II) HE staining: the structure of penile tissue was normal in normal control group, penile tissue of abnormal balgam syndrome model group was minor edema and interstitial fibrosis, abnormal balgam syndrome with impotence disease model group showed significant edema and interstitial fibrosis, morphological changes of penile tissue repaired partly in medication intervention group of abnormal balgam syndrome with impotence disease model. (III) VG staining: the ratio of smooth muscle/collagen fibers in penile tissue of abnormal balgam syndrome model group was significantly lower than normal control group (P<0.05). Abnormal balgam syndrome with impotence disease model group was lower than abnormal balgam syndrome model group (P<0.05). Medication intervention group of abnormal balgam syndrome with impotence disease model was higher than abnormal balgam syndrome with impotence disease model group (P<0.05). (IV) Masson staining results was consistent with VG staining. (V) Sexual hormones: compared with normal control group, testosterone was lower in abnormal balgam syndrome group and abnormal balgam syndrome with ED group (P<0.05); compared with abnormal balgam syndrome group with ED group; testosterone increased in medication intervention group of abnormal balgam syndrome with impotence (P<0.05). Compared with normal control group, estradiol, prolactin, luteinizing hormones was higher in abnormal balgam syndrome group and abnormal balgam syndrome with impotence group (P<0.05); compared with abnormal balgam syndrome group with ED group, estradiol, prolactin, luteinizing hormones increased in medication intervention group of abnormal balgam syndrome with impotence group (P<0.05). The results suggested that erectile function of abnormal balgam syndrome with impotence group decreased significantly, morphological changes occurred in penile tissue, and sexual hormones changes greatly, Yimusake can probably improve the erectile function by repair the morphological changes and regulate the sexual hormones level.

Keywords: Erectile function; balgam syndrome; gonad axial