Objective: To culture and myogenic differentiation of rat bone mesenchymal stem cells (BMSCs) in vitro, and provide the available seed cells for erectile dysfunction (ED) therapy.
Methods: Rat BMSCs were isolated and cultured from the femur and tibia of Sprague Dawley (SD) rat. Mesenchymal stem cell positive cellular markers CD49d, CD73, CD90, CD105, CD106 and negative cellular markers CD31, CD34 and CD45 were arrayed by flow cytometry analysis. Furthermore, the fourth passage cells were induced and identified by their capacities in the myogenic differentiation.
Results: The cultured cells expressed mesenchymal stem cell positive cellular markers CD49d, CD73, CD90, CD105 and CD106, and lacked negative cellular markers CD31, CD34 and CD45. Myogenic differentiation cells can be stained with alpha-smooth muscle actin (α-SMA) and desmin, respectively.
Conclusions: Rat BMSCs have been successfully isolated, cultured and myogenic differentiation in vitro. They could be used as autogenous BMSCs and gene modified BMSCs for ED therapy.
